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Home Methods and Protocols Analysis Desalting RNA oligomers

Desalting RNA oligomers

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Depending on the size of the RNA oligomers one of the following methods can be chosen:

1. HPLC with a Triethyl-amine-acetate (TEAA) buffer

Column: Reverse Phase Column (RP18 = C18)


A: A mixture of Triethyl-amine and acedic acid (100%) in 1:1 ratio, adjusted to pH 7.0 with trietylamine or acedic acid. Final concentration 0.1M

B: 25% Acetonitrile in eluent A.


2. Sephadex G-25 column

3. Ion exchange beads

4. Dialysis  (the right cut-off size must be chosen !!)